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Regulation of Plant Pores by MicroRNAs

Researchers in Daegu Gyeongbuk Institute of Science and Technology found environmental cues trigger microRNAs to regulate the development and distribution of tiny pores involved in photosynthesis in plants.

Plant pores, called stomata, are tiny openings mainly found on the surfaces of leaves. They are bordered by two ‘guard cells’, and are involved in gas exchange and water loss between plants and the atmosphere.

There is a good understanding of many molecular signals involved in turning on and off genes responsible for transforming stem cells to guard cells. In addition to normal hereditary processes, environmental triggers can also affect stomata development and distribution. For example, pathogen infections, high carbon dioxide levels and high temperatures lead to reduced pore density.

Molecular cell biologist June Kwak of the Daegu Gyeongbuk Institute of Science and Technology (DGIST) and colleagues investigated if RNA segments called micro RNAs (miRNAs) were involved in stomatal formation and distribution triggered by surrounding conditions. miRNAs are gene regulators that give multi-cellular organisms a degree of flexibility to respond and adapt to environmental changes.

June Kwak and his team developed an approach that allowed them to identify which miRNAs turned on throughout the transition from stem cell to guard cell. They found that slightly more than half of all known miRNAs in a plant called Arabidopsis activated during the various stages of guard cell development. Altering the expression of the miRNAs changed guard cell formation and distribution, indicating that miRNAs play a crucial role in the pore development.

Importantly, they singled out a miRNA, called miR399, for its involvement in controlling the pattern of stomatal pores. miR399 is known to regulate phosphate transporter proteins inside plant cells, suggesting a link between stomatal development and phosphate homeostasis in plants.

“This study reveals that miRNAs constitute a crucial component of stomatal development and control,” says Kwak. “Further research will help identify previously unknown cellular processes controlling stomatal development. We expect our findings will help provide a strategy for improving crop productivity by adjusting stomatal pore density, thereby effectively controlling photosynthesis in response to environmental changes.” [APBN]